| Primer Name | Specificity | Primer Sequence (5’ to 3’) | Specific Binding Location | Melting Temp (˚C) |
| A24SEQN | Exon 2 of HLA-A | CCCCGCTTCATCGCA | Codon 24 | 56.3 |
| A82SEQR | Exon 2 of HLA-A | TCTGGTTGTAGTAGCCGC | Codon 82 | 57.3 |
| A97SEQ | Exon 3 of HLA-A | TTCTCACACCGTCCAGAG | Codon 97 | 57.3 |
| A99SEQ | Exon 3 of HLA-A | CACACCATCCAGATGATGTA | Codon 99 | 55.8 |
| A114SEQN | Exon 3 of HLA-A | CTCCGCGGGTACCA | Codon 114 | 55.9 |
| A116SEQ | Exon 3 of HLA-A | GCGGGTACCACCAGT | Codon 116 | 59.2 |
| A70SSP1 | Exon 2 of HLA-A | CTCTCGGTCAGTCTGTGAG | Codon 70 | 59.7 |
| A70SSP2 | Exon 2 of HLA-A | CTCTCGGTCAGTCTGTGAC | Codon 70 | 59.7 |
| A77SSP1 | Exon 2 of HLA-A | GCGCAGGGTCCCCAGGTC | Codon 77 | 66.4 |
| A77SSP2 | Exon 2 of HLA-A | GCGCAGGGTCCCCAGGCT | Codon 77 | 66.4 |
| B23SEQN | Exon 2 of HLA-B | AGCCCCGCTTCATC | Codon 23 | 52.9 |
| B24SEQN | Exon 2 of HLA-B | AGCCCCGCTTCATCG | Codon 24 | 56.3 |
| B24SEQ2 | Exon 2 of HLA-B | AGCCCCGCTTCATCTCA | Codon 24 | 57 |
| B45SEQN | Exon 2 of HLA-B | CGCCAGTCCGAGGAT | Codon 45 | 56.3 |
| B83SEQN | Exon 2 of HLA-B | TCTGGTTGTAGTAGCC | Codon 83 | 51.5 |
| B94SEQ | Exon 3 of HLA-B | AGCGAGGCCGGGTCTCACAT | Codon 94 | 64 |
| B94SEQNS | Exon 3 of HLA-B | AGGCCGGGTCTCACAT | Codon 94 | 56.7 |
| B97SEQ | Exon 3 of HLA-B | GGTCTCACATCATCCAGA | Codon 97 | 55.0 |
| B99SEQ | Exon 3 of HLA-B | ACCCTCAGAGGATGTAC | Codon 99 | 54.6 |
| B99SEQ2N | Exon 3 of HLA-B | TCACATCCAGAGGATGTAT | Codon 99 | 53.2 |
| B116SEQN | Exon 3 of HLA-B | CGGGCATGACCAGTA | Codon 116 | 53.6 |
| B116SEQ2N | Exon 3 of HLA-B | GCGGGCATAACCAGTA | Codon 116 | 54.1 |
| B156SEQN | Exon 3 of HLA-B | TCCAGGTAGGCTCTCC | Codon 156 | 56.7 |
| B163SEQRN | Exon 3 of HLA-B | CCACTCCACGCACTC | Codon 163 | 56.3 |
| B45SEQN | Exon 2 of HLA-B | GCCGCGAGTCCGAGGAT | Codon 45 | 61.8 |
| C35SEQ | Exon 2 of HLA-C | ACGACACGCAGTTCGTGCG | Codon 35 | 61.9 |
| C43SEQ | Exon 2 of HLA-C | GACGCCGCGAGTCCG | Codon 43 | 61.8 |
| C129SEQR | Exon 3 of HLA-C | GGTCCAGGAGCGCAGG | Codon 129 | 61.8 |
| C24SEQ | Exon 2 of HLA-C | GAGCCCCGCTTCATCT | Codon 24 | 56.7 |
| DPA15SEQ | Exon 2 of HLA-DPA1 | TATGCCGCGTTTGTACAGACC | Codon 15 | 60.0 |
| DPA15SEQRV | Exon 2 of HLA-DPA1 | TATGCCATGTTTGTACAGACC | Codon 15 | 56.1 |
| DPB11SEQ | Exon 2 of HLA-DPB1 | AGAATTACCTTTTCCAGG | Codon 11 | 50.5 |
| DPB36SEQ | Exon 2 of HLA-DPB1 | ACCGGGAGGAGTTCGC | Codon 36 | 59.2 |
| DPB36SEQRV | Exon 2 of HLA-DPB1 | AACCGGGAGGAGTTCGT | Codon 36 | 57.0 |
| DPB76SEQR | Exon 2 of HLA-DPB1 | CTCGTAGTTGTGTCTGCAT | Codon 76 | 55.4 |
| DQB9SEQ | Exon 2 of HLA-DQB1 | CGCAGAGGATTTCGTGTA | Codon 9 | 55.0 |
| DQB0201F | Exon 2 of HLA-DQB1 | GTGCGTCTTGTGAGCAGAAG | Codon 30 | 59.9 |
| DQB0301F | Exon 2 of HLA-DQB1 | GGACGGAGCGCGTGCGTT | Codon 26 | 64.1 |
| DRB58SEQN | Exon 2 of HLA-DRB1 | TGGGGCGGCCTGATGA | Codon 58 | 59.2 |
| DRB86SEQ | Exon 2 of HLA-DRB1 | ACTGTGAAGCTCTCAC | Codon 86 | 51.5 |